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Family Tree DNA Reconstuction Project

When I started this project in the late 1990's, I had hoped it would magically tie relatives of the 5 brothers together to prove that they were in fact brothers. But, that didn't happen. Instead it left me with more questions. To date seventy Middleton's have participated in the DNA project. Below I've listed five project members who have connections with our line in SC, that migrated to either AL or MS. All 5 have taken at least the 25-marker test and those results are in the table below. The last two columns of the table shows the 12-marker and 25-marker distance from my results.

John W. Middleton - Kit number 2681. My assumption is that my Middleton line is from England, but it might be more safe to state it as the British Isles. I feel pretty strongly that my line was in South Carolina by the 1750's. My verified Middleton line starting with my grandfather is: William Lafayett b. 1867 AL, Henry H. b. 1834 AL, Jeptha b. 1812 SC. I believe that Jeptha's father is Martin Middleton b. circa 1770 SC, but have no definitive proof,. but on 7/30/02 I got a match with Subject 5. His line is from one of the other "5 brothers" that we think are descendant from Martin. I match with subjects 5, 6, 26 and 29 on the 12-marker test.

Bunny L. Middleton - Kit number 3688. Acting as point of contact for Bunny is his nephew, Cecil Catrett. Our verified line starting with Bunny's father is Elbert, b. May 1910 in Monroe Co., AL, Samuel, b. Dec. 1882 in Monroe Co., AL, John G., b. abt. April 1822 in AL, Willis, b. 1797 in SC. We believe that Willis is probably the son of Martin and Pemmy Middleton of Marion Co., SC and that the aforementioned Pemmy is the same Pemmy listed on the 1850 Monroe Co., AL Census (age 80) living in Willis' household. I glad that we now have definitive proof of kinship between Willis and Jeptha. (Note: "Pemmy" is one of many references found for Penina Foxworth, b. abt. 1770 in SC.) Bunny matches with subjects 1, 6, 26 and 29 on the 12 marker test.

Roger Lewis Middleton - Kit number 3960 - Roger's results were posted 8/15/02, and we have another match! He matches with Subjects 1 and 5; thus, helping to link the "5 brothers" from SC. Bill Crider is acting as the contact for his Uncle Roger Lewis Middleton. Roger's line is as follows: Unknown (Maybe Martin?)>Stephen Middleton, b. 1846 AL -> Elbert Earl Middleton b. 1870 AL -> Faye Egan Middleton b. 1907 LA -> Roger Lewis Middleton (living.) Roger is my mother's brother. I have a lot more information - still needs organizing. (Note: The 1850 AL census shows Martin Middleton with a son named Stephen. The listed Stephen is 2 years old, but Bill's Stephen should be 4 years old. This is a prime example of how census data can throw off the researcher.) Roger matches with subjects 1, 5, 26 and 29 on the 12-marker test.

Charles Volney Middleton - Kit number 23548 - This kit was returned on 8/4/04. Benton Fletcher (Email) is the contact for this line. I have been aware that Benton has been researching Middletons in Miss. Terr. for years along with Pauline Hallett. Well, the results are back and Charles matches the 3 of us in my line! :) This proves what we suspected; that our line did go to Franklin Co, MS, in the early 1800's. I believe this to include a John, Willis and Stephen Middleton. Later, in about 1821, the rest of the family moved from SC, to AL. The two parts of the line seemed to lose contact. **NOTE** Another Middleton who traces back to MS (Sub 29), matched our group 12/12, but missed by 7 on the 25-marker test. So, I would highly suggest that Charles upgrades to determine if he has similar results.

His line starts with Stephen J. MIDDLETON was b.abt. 1805 in AL; d.BEF 1850 in Franklin Co., MS. Married Celia Ann King LONG ABT 1824. Celia b. 4 Jul 1808, Montgomery Co., Tn.; d. 1860, Franklin Co., MS.

Charles E. Middleton - Kit number 26615 - The kit was returned and 25-marker results were posted 3/10/05. Benton Fletcher (Email) is the contact for this line as he is for Subject 26. This is a weird one! Charles matches 12/12 with my line (Subjects 1, 5, 6 & 26.) But, he upgraded to the 25-marker test and he and I are off by 6 markers. See discussion section below for some comments.

Results of the 25-Marker DNA Analisys for the 5 men above:

Sub# MigraRt Loc01 Loc02 Loc03 Loc04 Loc05 Loc06 Loc07 Loc08 Loc09 Loc10 Loc11 Loc12 Loc13 Loc14 Loc15 Loc16 Loc17 Loc18 Loc19 Loc20 Loc21 Loc22 Loc23 Loc24 Loc25 12D 25D
#1 SC>AL 13 24 15 11 11 14 12 12 13 13 13 29 17 09 10 11 11 25 15 20 31 15 15 17 17 - -
#5 SC>AL 13 24 15 11 11 14 12 12 13 13 13 29 17 09 10 11 11 25 15 20 31 15 15 15 17 0 1
#6 SC>MS 13 24 15 11 11 14 12 12 13 13 13 29 17 09 10 11 11 25 15 20 32 14 15 17 17 0 2
#26 SC>MS 13 24 15 11 11 14 12 12 13 13 13 29 17 09 10 12 11 25 15 20 31 15 15 15 17 0 2
#29 SC>MS 13 24 15 11 11 14 12 12 13 13 13 29 18 09 10 11 11 24 15 19 29 15 16 17 18 0 6